Fig 2: A. m7G score-hub gene network for the top 5 most highly regulated genes. B. Combination pattern diagram of Bleomycin and EIF4E. Yellow represents hydrogen bonding, and Amino acid residue includes ALA229, HIS228, ASN72, SER85, ARG87, ILE89, ASP71, ASP116, LYS183, and LYS138. C. Combination pattern diagram of Etoposide and EIF4E2. Yellow represents hydrogen bonding, Amino acid residue includes SER24, THR22, SER64, and THR99. D. Combination pattern diagram of Bleomycin and EIF4E3. Yellow represents hydrogen bonding, Amino acid residue includes ARG152, LEU83, ALA49, GLU93, ARG95, HIS194, and LYS192. E. Combination pattern diagram of Bleomycin and NCBP1. Yellow represents hydrogen bonding; Amino acid residue includes LYS650, ARG610, ARG646, GLN753, ASP369, LYS455, ARG458, and GLN599. F. Combination pattern diagram of Etoposide and NCBP2. Yellow represents hydrogen bonding, Amino acid residue includes ARG227, ARG104, VAL126, and ARG119

Fig 3: A–B Western blot experiment highlights the expression profile of NCBP1, NCBP2, EIF4E, EIF4E2, and EIF4E3 proteins in a total of ten tissue samples of LGG and ten healthy brain tissues. C–M Relative expression levels of NCBP1, NCBP2, EIF4E, EIF4E2, and EIF4E3 (five potentially prognostic m7G regulatory proteins) in ten LGG tissues and ten normal brain tissues. GAPDH was utilized as a loading control. The values were normalized by log2 fold change (ratio of tumor to healthy tissue expression) of the target proteins